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  • Optimize your Sample, Optimize your Research – The Basics in Sample Preparation
    June 24th Tuesday June 25th Wednesday June 26th Program as PDF Speakers List Young Investigator Awards Sponsors Training Courses Vendor Seminars Postersession Training Courses Thursday June 27 th Friday June 28 th Hands On Workshop 9 00 12 30h 13 30 16 00h Register online Stefan Loroch Yvonne Pasing Optimize your Sample Optimize your Research The Basics in Sample Preparation In large scale bottom up proteomics success and fail critically depends on the quality of sample preparation prior LC MS analysis Lysis techniques subsequent protein purification steps and setting up the right conditions for digest have to be carefully optimized to generate a maximum of specific peptides while generation of non specific peptides must be avoided Desalting of peptides afterwards is essential to maximize your protein IDs and can be easily combined with peptide prefractionation using different SPE materials In this workshop methods for proper sample preparation will be learned including protein purification protein digest and quality control via monolithic HPLC systems Furthermore advantages and proper usage of different SPE materials for desalting and prefractionation will be discussed The practical part will cover the following techniques Purification and Tryptic Digest Protein precipitation and in solution digest Filter aided sample preparation

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/55-training-course-loroch-pasing (2016-04-30)
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  • Enrich your peptides of N-terest
    27 th Friday June 28 th Hands On Workshop 9 00 12 30h 13 30 16 00h Register online Chris Gaevert Evy Timmermann An Staes Enrich your peptides of N terest N terminal COFRADIC is a very powerful technique to enrich for N terminal peptides of proteins This technique has lifted the study of protease substrates and protein N terminal modifications to a higher level In this workshop you will

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/54-trainings-course-gaevert-timmermann-staes (2016-04-30)
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  • Hands-on MRM Workshop
    9 00 12 30h 13 30 16 00h Register online Dominik Domanski Christoph Borchers Hands on MRM Workshop This hands on workshop will cover the general principles of MRM analysis including following topics Assay development techniques Internal isotopic standard peptides Selection of target peptides Synthesis of standard peptides Optimization of MS parameters Sample preparation Validation of specificity Balancing of endogenous to standard peptide Determining the limit of detection quantification Determining

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/53-trainings-course-smith-borchers (2016-04-30)
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  • Workshop I - Utilizing Nano UHPLC to Maximize Peptide and Protein Identification Workshop II – High Resolution 2D-LC of Proteins and Peptides
    Einladung Program Monday June 24th Tuesday June 25th Wednesday June 26th Program as PDF Speakers List Young Investigator Awards Sponsors Training Courses Vendor Seminars Postersession Training Courses Thursday June 27 th Friday June 28 th Hands On Workshop 9 00 12 30h 13 30 16 00h Register online Steffen Liedtke Sebastiaan Dolman Workshop I Utilizing Nano UHPLC to Maximize Peptide and Protein Identification Thursday In the theory session the following topics are covered introduction nano LC maximizing peak capacity in 1D and 2D LC MS coupling aspects of nano LC columns to MS and introduction of EasySpray In addition some common diagnostics and troubleshooting will be presented The hands on session covers the following topics installation of nano LC columns working with nanoViper programming optimal gradient conditions for 1D separations reviewing high resolution nano LC data Workshop II High Resolution automated 2D LC of Peptides Friday In the theory session the following topics are covered introduction PS DVB monolithic columns challenges in protein separations maximizing peak capacity in 2D LC MS employing monolithic columns automated fractionation capabilities of the autosampler on line vs off line 2D LC techniques The hands on session covers the following topics installation of monolithic columns

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/52-training-course-liedtke-sneekes-swart (2016-04-30)
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  • Phosphorylation Analysis
    9 00 12 30h 13 30 16 00h Register online Rene Zahedi and Sonja Radau Phosphorylation Analysis Under supervision 4 5 participants will prepare process and analyze phosphoproteomics samples Cell lysates will be proteolytically digested and processed phosphopeptides will be enriched and analyzed by nano LC MS MS using state of the art instrumentation Participants will learn about current quality control issues in phosphoproteomics such as correct site localization and

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/51-trainings-course-zahedi (2016-04-30)
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  • Preparation and label free and label-based Quantification of Membrane Proteins
    free and label based Quantification of Membrane Proteins One of the most important methods in order to identify changes in cellular signaling pathways and disease specific biomarkers is the relative and absolute quantitation of protein abundances Many diseases such as Morbus Alzheimer are membrane protein dependent disorders and quantifying abundances of membrane proteins is a fundamental step in understanding the underlying pathomechanisms In the first part of the workshop we demonstrate a label free quantification approach based on 1D gel sample separation followed by LC MS MS analysis and relative protein quantification spectral counting Furthermore the participants will improve their skills regarding the isolation of membrane proteins Therefore we will focus on two different subclasses of common used detergents the anionic surfactant SDS and the non ionic glycoside digitonin While SDS triggered membrane preparation results in a high output of free membrane proteins the presence of the mild non ionic detergent digitonin allows the preparation of native membrane protein complexes In the second part of the workshop strengths and weaknesses of absolute quantification strategies for membrane proteins will be discussed based on self conducted experiments For this purpose an integral membrane protein will be quantified with AQUA as well as

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/50-training-course-poetsch-schroetter-magraoui (2016-04-30)
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  • MALDI-Imaging
    Young Investigator Awards Sponsors Training Courses Vendor Seminars Postersession Training Courses Thursday June 27 th Friday June 28 th Hands On Workshop 9 00 12 30h 13 30 16 00h Register online Corinna Henkel Hanna Diehl Michael Becker Herbert Thiele Dennis Trede MALDI Imaging Biomolecular imaging MS allows the researcher to analyze the spatial distribution of biomolecules such as proteins peptides lipids or even drugs and their metabolites in tissue sections and therby covering a wide field of applications Beyond that the technique requires no labeling allows the simultaneous mapping of multiple analytes over the full mass range and in combination with histology one can obtain distinct histopathological features Latter one can be used within the biomarker discovery pipeline This hands on workshop will provide newcomers to the field of MALDI Imaging with the necessary background knowledge and the practice for carrying out different imaging experiments Proteins peptides and lipids are planned to be processed Automated matrix application and measurements will be carried out on Bruker instruments and the vendor will also be present to support subsequent data analysis including statistical analysis In addition an alternative software solution from the Steinbeis Innovationszentrum SCiLS Scientific Computing in Life Sciences will be

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/49-training-course-henkel-diehl-becker-thiele-trede (2016-04-30)
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  • Hands-on Workshop Redox-Proteomics
    00h Register online Alexandra Müller and Lars Leichert Redox Proteomics This hands on workshop will cover the general principles of MS based redox proteomics using a model protein The workshop is aimed at proteome researchers with an interest in oxidative stress and redox biology Oxidative stress is the inevitable consequence of an aerobic life style Reactive oxygen species the unwanted by products of many essential cellular processes such as respiration can damage virtually all important bio molecules Therefore it is not surprising that many pathological conditions are associated with oxidative stress and that on a molecular level reactive oxygen species are thought to be the causative agent of cellular ageing While these destructive properties of oxidative stress have long been known more recently it became apparent that so called redox regulated proteins are not damaged by oxidative stress but instead use the amino acid cysteine as a nano switch to sense the redox environment of the cell In the last few years quantitative proteomic methods have been developed to identify these redox sensitive cysteines and to study cysteine oxidation under pathological and physiological stress conditions on a global level Here we will cover the basic principles of these methods and

    Original URL path: http://www.arbeitstagung.de/index.php/2013trainingcourses/48-training-course-mueller-leichert (2016-04-30)
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